Proteomic identification of rainbow trout blood plasma proteins and their relationship to seminal plasma proteins
PBN-AR
Instytucja
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Proteomics (35pkt w roku publikacji)
ISSN
1615-9853
EISSN
1615-9861
Wydawca
DOI
URL
Rok publikacji
2017
Numer zeszytu
11
Strony od-do
art. no 1600460 (1-15)
Numer tomu
17
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
2D DIGE
blood plasma
fish
mass spectrometry
seminal plasma
Streszczenia
Język
en
Treść
The characterization of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study LC-MS/MS and 2D DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified in SP are involved in a variety of signalling pathways, including protein ubiquitination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X receptor (FXR)/RXR activation, cell cycle and acute phase signalling. These findings may indicate the prevalence of acute phase signalling pathways in trout SP, and its essential role in protecting spermatozoa and reproductive tissues. Our study provides the first in-depth analysis of the trout BP proteome, with a total of 119 proteins identified. The major proteins of rainbow trout BP were recognized as acute phase proteins. Analysis of BP proteins indicated that acute phase response signalling, the complement system, LXR/RXR and FXR/RXR activation and the coagulation system are the top canonical pathways. This study enhances knowledge of the blood origin of trout SP proteins and understanding of fish reproductive biology. Our results provide new insight into blood proteins specifically important for fish physiology and innate immunity. The mass spectrometry data are available via ProteomeXchange with the identifier PXD005988 and https://doi.org/10.6019/PXD005988.
Inne
System-identifier
252-001267
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