Evaluation of miR-506 and miR-4316 expression in early and non-invasive diagnosis of colorectal cancer
PBN-AR
Instytucja
Narodowy Instytut Onkologii im. Marii Skłodowskiej-Curie – Państwowy Instytut Badawczy
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
English
Czasopismo
International Journal of Colorectal Disease (30pkt w roku publikacji)
ISSN
0179-1958
EISSN
1432-1262
Wydawca
DOI
URL
Rok publikacji
2017
Numer zeszytu
7
Strony od-do
1057-1060
Numer tomu
32
Identyfikator DOI
Liczba arkuszy
0.5
Słowa kluczowe
en
Colorectal cancer
Early diagnosis and prevention
microRNAs
Liquid biopsy
Streszczenia
Język
en
Treść
Examination of the entire colon by colonoscopy remains the golden standard for screening of colorectal cancer (CRC). However, patients are reluctant to perform invasive colonoscopies because of interference with their intimacy. Therefore, the potential use of non-invasive analysis of microRNAs expression in liquid biopsy as a novel biomarker for early CRC has investigated in several studies. In this study, we analyzed the expression of two novel microRNAs: miR-506 and miR-4316, which have never been examined in CRC. Plasma samples were collected from 56 patients (median age of 68 years) with operable colorectal cancer and from 70 healthy individuals (median age of 59 years). Expression of plasma microRNAs was evaluated by quantitative reverse transcription polymerase chain reaction using Eco real-time PCR device (Illumina, USA). We found a significant elevated expression of both examined microRNAs in early CRC patients when compared to those in healthy individuals (p = 0.0054 for miR-506 and p = 0.0025 for miR-4316). The expression of miR-506 and miR-4316 did not depend on gender, age, disease stage, and tumor localization of CRC patients. ROC curve analysis showed that both examined microRNAs could differentiate early stage colorectal cancer from healthy individuals with 76.8% specificity and 60.7% sensitivity for miR-506 analysis and 76.8% specificity and 75% specificity for miR-4316 analysis. Our study revealed that elevated expression of miR-506 and miR-4316 in peripheral blood were potential molecular markers for early colorectal cancer.
Inne
System-identifier
COI556de7b9bfe94bba8d5f065c4a05d3ec
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