Salicylic acid is an indispensable component of the Ny-1 resistance-gene mediated response against Potato virus Y infection in potato
PBN-AR
Instytucja
Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Experimental Botany
ISSN
0022-0957
EISSN
1460-2431
Wydawca
Oxford University Press
DOI
Rok publikacji
2014
Numer zeszytu
4
Strony od-do
1095–1109
Numer tomu
65
Identyfikator DOI
Liczba arkuszy
Streszczenia
Język
en
Treść
The purpose of the study was to investigate the role of salicylic acid (SA) signalling in Ny-1 -mediated hypersensitive resistance (HR) of potato ( Solanum tuberosum L.) to Potato virus Y (PVY). The responses of the Ny-1 allele in the Rywal potato cultivar and transgenic NahG-Rywal potato plants that do not accumulate SA were characterized at the cytological, biochemical, transcriptome, and proteome levels. Analysis of noninoculated and inoculated leaves revealed that HR lesions started to develop from 3 d post inoculation and completely restricted the virus spread. At the cytological level, features of programmed cell death in combination with reactive oxygen species burst were observed. In response to PVY infection, SA was synthesized de novo . The lack of SA accumulation in the NahG plants led to the disease phenotype due to unrestricted viral spreading. Grafting experiments show that SA has a critical role in the inhibition of PVY spreading in parenchymal tissue, but not in vascular veins. The whole transcriptome analysis confirmed the central role of SA in orchestrating Ny-1 -mediated responses and showed that the absence of SA leads to significant changes at the transcriptome level, including a delay in activation of expression of genes known to par - ticipate in defence responses. Moreover, perturbations in the expression of hormonal signalling genes were detected, shown as a switch from SA to jasmonic acid/ethylene signalling. Viral multiplication in the NahG plants was accompa - nied by downregulation of photosynthesis genes and activation of multiple energy-producing pathways.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
PX-56988501810641ecf918ed8a
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