A comparison of two methods of determining colour change in the assessment of the quality of pork
Wydział Biotechnologii i Hodowli Zwierząt (Zachodniopomorski Uniwersytet Technologiczny w Szczecinie)
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ACTA Scientiarum Polonorum Technologia Alimentaria (15pkt w roku publikacji)
Uniwersytet Przyrodniczy w Poznaniu
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meat quality
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The colour of pork is one of the most important measures of meat quality. For the consumer, it is a sign of freshness, crucial for the decision to buy a product. The storage of meat results in constant colour changes. Differences can be found in all colour parameters, especially chromatic ones, such as redness (a*) and hue angle (h°), as these changes are significantly correlated with a number of quality traits. Colour change can be determined not only by evaluating changes in colour parameters, but also by establishing the change in absorbance of specific wavelengths of light. The aim of this study was to compare the usefulness of these two methods of determining colour change: parameters measured on the CIELAB and CIELCh scales and colour change (%CC) determined based on the absorbance of selected wavelengths of light, i.e. 505, 540, 560, 580 and 630 nm. In this paper, we analysed two methods of measuring colour change in terms of their relevance for meat quality assessment. The study involved 150 samples of the longissimus lumborum muscle collected from 150 carcasses of pigs slaughtered on an industrial line, weighing 65–107 kg, with an average carcass weight of 86.1 kg. Meat sensory analysis (colour, wateriness, firmness), physicochemical assessment (colour, WHC, pH48) and proximate analysis (moisture, total protein, fat, ash) were carried out. The methods included the determination of (I) changes in colour parameters measured on the CIELAB and CIELCh scales, and (II) colour change (%CC) based on the absorbance of selected wavelengths: 505, 540, 560, 580 and 630 nm. Chromatic absorbance values of a wavelength of 525 nm (A525p) and the relative content of Mb, MbO2 and MetMb were calculated according to the method proposed by Krzywicki (1979). Basic chemical composition – with few exceptions – was not significantly correlated with differences in the values of colour parameters (ΔL*, Δa*, Δb*, ΔC*, Δh°) and colour change (%CC). With an increase in scores in the sensory evaluation of colour, wateriness, and firmness, and an increase in WHC and pH48, the differences in lightness (ΔL*) significantly increased and differences in chromatic value (Δa*, Δb*, ΔC*, Δh°) and %CC decreased, for all tested combinations of wavelengths. The highest correlation coefficients were observed for Δa* and Δh° and %CC540/630 and %CC580/630, with colour change (%CC540/630 and %CC580/630) showing a stronger correlation with the tested meat quality traits than the differences in redness (Δa*) and hue angle (Δh°). We found a very strong correlation between changes in the relative levels of MetMb (ΔMetMb) and colour change (%CC) based on the absorbance of wavelengths of 540 and 505 nm, and 580 and 505 nm (r = 0.950*** and r = 0.967***, respectively). The determination of colour change based on the absorbance of two combinations of wavelengths, i.e. (I) 540 and 630, and (II) 580 nm and 630 nm, is more useful in the assessment of pork quality than changes in two most relevant parameters of the CIELAB and CIELCh scales, i.e. redness (Δa*) and hue angle (Δh°). Moreover, the determination of absorbance of a wavelength of 630 nm was more useful for determining colour change (%CC) than 505 nm. Colour change (%CC) based on the absorbance of wavelengths of 540 and 505 nm and 580 and 505 nm reflect the changes in the relative amounts of MetMb very well.
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