DNA metabarcoding study of a polyphagous beetle dietary diversity: the utility of barcodes and sequencing techniques
PBN-AR
Instytucja
Instytut Systematyki i Ewolucji Zwierząt Polskiej Akademii Nauk
Źródłowe zdarzenia ewaluacyjne
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
Folia Biologica (Kraków)
ISSN
0015-5497
EISSN
1734-9168
Wydawca
DOI
URL
Rok publikacji
2014
Numer zeszytu
3
Strony od-do
223-234
Numer tomu
62
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 1)
Streszczenia
Język
angielski
Treść
Recently developed techniques ofDNAbarcoding and next-generation sequencing (NGS) overcome previous limitations of evaluation of animal diet composition and together are promising method in molecular ecology. The objective was to compare standard ABI Sanger sequencing with new high throughput sequencing (Illumina MiSeq) technique and the two selected plant barcodes (rbcL gene and trnL intron) in terms of the identification of host plant composition for the selected beetle species – the Centricnemus leucogrammus weevil. A comparison of two sequencing techniques showed that NGS (in this case Illumina) gave more exhaustive results than the Sanger method. Moreover, it was proven that a two-locus barcoding systems (rbcL and trnL) is sufficient for host plant identification from DNA isolated from insect bodies, at least at the genus level. A comparison of host plant composition among distant populations revealed that the studied species did not feed uniformly across its range. This probably reveals an ecological adaptation of geographically and genetically isolated populations. These findings, beside broadening basic knowledge on the use of barcoding and sequencing techniques for host plant identifications in insect populations, can have implications for conservation studies and strategies for rare and endangered species.
Inne
System-identifier
PX-56b474718106eb71826ed47c
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