Development of somatic hybrids Solanum x michoacanum Bitter.(Rydb.)(+) S. tuberosum L. and autofused 4x S. x michoacanum plants as potential sources of late blight resistance for potato breeding
PBN-AR
Instytucja
Instytut Hodowli i Aklimatyzacji Roślin - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
PLANT CELL REPORTS
ISSN
0721-7714
EISSN
1432-203X
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2013
Numer zeszytu
8
Strony od-do
1231-1241
Numer tomu
32
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Potato species – Protoplast electrofusion – Phytophthora infestans resistance – Somatic hybridization
Streszczenia
Język
en
Treść
Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2n = 2x = 24) potato species derived from spontaneous cross of S. bulbocastanum and S. pinnatisectum. This hybrid is a 1 EBN (endosperm balance number) species and can cross effectively only with other 1 EBN species. Plants of mch are resistant to Phytophthora infestans (Mont) de Bary. To introgress late blight resistance genes from mch into S. tuberosum (tbr), genepool somatic hybridization between mch and susceptible diploid potato clones (2n = 2x = 24) or potato cultivar Rywal (2n = 4x = 48) was performed. In total 18,775 calli were obtained from postfusion products from which 1,482 formed shoots. The Simple Sequence Repeat (SSR), Cleaved Amplified Polymorphic Sequences (CAPS) and Random Amplified Polymorphic DNA (RAPD) analyses confirmed hybrid nature of 228 plants and 116 autofused 4x mch. After evaluation of morphological features, flowering, pollen stainability, tuberization and ploidy level, 118 somatic hybrids and 116 autofused 4x mch were tested for late blight resistance using the detached leaf assay. After two seasons of testing three somatic hybrids and 109 4x mch were resistant. Resistant forms have adequate pollen stainability for use in crossing programme and are a promising material useful for introgression resistance from mch into the cultivated potato background.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
586897
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