Norepinephrine transporter knock-out alters expression of the genes connected with antidepressant drugs action
PBN-AR
Instytucja
Instytut Farmakologii im. Jerzego Maja Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
BRAIN RESEARCH
ISSN
0006-8993
EISSN
1872-6240
Wydawca
ELSEVIER
DOI
Rok publikacji
2015
Numer zeszytu
Strony od-do
284-292
Numer tomu
1594
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Norepinephrine transporter knock-out mice;
TaqMan low density array;
Microdissection;
Cortex;
Hippocampus;
Amygdala
Streszczenia
Język
en
Treść
Norepinephrine transporter knock-out mice (NET-KO) exhibit depression-resistant phenotypes. They manifest significantly shorter immobility times in both the forced swim test and the tail suspension test. Moreover, biochemical studies have revealed the up-regulation of other monoamine transporters (dopamine and serotonin) in the brains of NET-KO mice, similar to the phenomenon observed after the chronic pharmacological blockade of norepinephrine transporter by desipramine in wild-type (WT) animals. NET-KO mice are also resistant to stress, as we demonstrated previously by measuring plasma corticosterone concentration. In the present study, we used a microdissection technique to separate target brain regions and the TaqMan Low Density Array approach to test the expression of a group of genes in the NET-KO mice compared with WT animals. A group of genes with altered expression were identified in four brain structures (frontal and cingulate cortices, dentate gyrus of hippocampus and basal-lateral amygdala) of NET-KO mice compared with WT mice. These genes are known to be altered by antidepressant drugs administration. The most interesting gene is Crh-bp, which modulates the activity of corticotrophin - releasing hormone (CRH) and several CRH-family members. Generally, genetic disturbances within noradrenergic neurons result in biological changes, such as in signal transduction and intercellular communication, and may be linked to changes in noradrenaline levels in the brains of NET-KO mice.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
PX-56989cd5810641ecf9198efd
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