Mesolimbic dopamine D receptor plasticity contributes to stress resilience in rats subjected to chronic mild stress
PBN-AR
Instytucja
Instytut Farmakologii im. Jerzego Maja Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
Psychopharmacology
ISSN
0033-3158
EISSN
1432-2072
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2013
Numer zeszytu
4
Strony od-do
583-593
Numer tomu
227
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Chronic mild stress;
Depression;
Domperidone;
D2 receptor;
Dopamine mesolimbic system;
In situ hybridisation;
mRNA;
Stress resilience;
Rat
Open access
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Creative Commons — Uznanie autorstwa
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Streszczenia
Język
en
Treść
Rationale Few studies have investigated neurobiological and biochemical differences between stress-resilient and stress-vulnerable experimental animals. Objectives We investigated alterations in mesolimbic dopamine D2 receptor density and mRNA expression level in stressed rats at two time points, i.e. after 2 and 5 weeks of chronic mild stress (CMS). Methods We used the chronic mild stress paradigm because it is a well-established animal model of depression. Two groups of stressed rats were distinguished during CMS experiments: (1) stress reactive (70 %), which displayed a decrease in the drinking of a palatable sucrose solution during the stress regimen, and (2) stress resilient (30 %), which exhibited an unaltered drinking profile when compared with the unchallenged control group. [3H]Domperidone was used as a ligand to label dopamine D2 receptors, and a mixture of three specific oligonucleotides was used to evaluate dopamine D2 receptor mRNA changes in various regions of the rat brain. Results CMS strongly affected the mesolimbic dopamine circuit in stress-resilient group after 2 weeks and stress-reactive group of rats after 5 weeks which exhibited a decrease in the level of dopamine D2 receptor protein without alterations in D2 mRNA expression. Stress-resilient animals, but not stress-reactive animals, effectively adapted to the extended stress and coped with it. The increase in D2 mRNA expression returned the dopamine D2 receptor density to control levels in stress-resilient rats after 5 weeks of CMS, but not in stress-reactive animals. Conclusions These results clearly demonstrate that, despite earlier blunting, the activation of dopamine receptor biosynthesis in the dopamine mesoaccumbens system in stress-resilient rats is involved in active coping with stressful experiences, and it exhibits a delay in time.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
480875
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