The structural and phylogenetic profile of the 3’ terminus of coxsackievirus B3 negative strand
PBN-AR
Instytucja
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
VIRUS RESEARCH
ISSN
0168-1702
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2014
Numer zeszytu
Strony od-do
81-89
Numer tomu
188
Identyfikator DOI
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Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
en
Coxsackievirus 83
Cloverleaf
RNA secondary structure
RNA structure probing
Negative-strand RNA
Negative viral strand
Streszczenia
Język
en
Treść
In the replication process of RNA(+) viruses both the positive-strand template and the newly synthesized negative strand appear in a double-stranded form, RF. It has been shown for poliovirus that prior to the initiation of positive-strand synthesis, the 5'-terminus of the positive strand must adopt a cloverleaf structure. When that happens, the 3'-terminal region of the negative strand is released from the RF form and is able to form into its own defined structure. In order to determine the secondary structure of this region, a comprehensive approach consisting of experimental mapping methods, phylogenetic analysis and computer predictions was applied. Here we propose the first structural model of the 3'-terminal region of the coxsackievirus B3 (CV-B3) negative strand, approximately 450 nucleotides in length. The region folds into three highly defined structural domains, The most 3'-terminal part of this region is domain I', which folds into a cloverleaf structure similar to that found in the viral RNA strand of positive-polarity. Remarkably, this motif is conserved among all analyzed viral isolates of CV-B3 despite the observed sequence diversity. Several other conserved structural motifs within the 3'-terminal region of the viral negative strand were also identified. The structure of this region may be crucial for the replication complex assembly.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
556282
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