Genetic Analysis of Poliovirus Strains Isolated From Sewage in Poland
PBN-AR
Instytucja
Narodowy Instytut Zdrowia Publicznego - Państwowy Zakład Higieny
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF MEDICAL VIROLOGY
ISSN
0146-6615
EISSN
1096-9071
Wydawca
WILEY-BLACKWELL
DOI
URL
Rok publikacji
2014
Numer zeszytu
7
Strony od-do
1243-1248
Numer tomu
86
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
OPV
recombinant
sequencing
environmental surveillance
polio eradication
Streszczenia
Język
en
Treść
The study describes genetic characterization of poliovirus (PV) strains isolated from sewage samples in Poland. The analyses were performed for the detection of any putative polio revertants and recombinants in three genomic regions by sequencing analysis. Thirty-six strains were analyzed. The analyzed strains were identified by neutralization assay as 7 strains of serotype P1, 10 strains of serotype P2, and 19 strains of serotype P3. Sewage isolates were sequenced in 5′UTR, VP1, and 3D genomic regions. All detected PVs were classified as vaccine strains on the basis of VP1 sequence. Mutational differences in the VP1 sequences of isolated viruses ranged from 0.0% to 0.4%, indicating a limited replication period. The genetic analysis of the 3D region showed that some strains have recombinant genomes. Nine strains were found as dipartite recombinants (seven strains—S3/S2, one strain—S2/S1, one strain—S3/S1), while one strain was found as tripartite recombinant (S3/S2/S1). No recombinants with non-PV enteroviruses were identified. None of wild-type PVs or vaccine-derived polioviruses (VDPVs) were detected. This study showed the absence of wild or VDPV circulation in the country and demonstrated the usefulness of environmental surveillance in addition to acute flaccid paralysis (AFP) surveillance in support of polio eradication initiatives
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
661956
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