The preferential nNOS inhibitor 7-nitroindazole and the non-selective one N-G-nitro-L-arginine methyl ester administered alone or jointly with L-DOPA differentially affect motor behavior and monoamine metabolism in sham-operated and 6-OHDA-lesioned rats
PBN-AR
Instytucja
Instytut Farmakologii im. Jerzego Maja Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Brain Research
ISSN
0006-8993
EISSN
1872-6240
Wydawca
ELSEVIER
DOI
Rok publikacji
2015
Numer zeszytu
Strony od-do
218-237
Numer tomu
1625
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Dopamine metabolism,
L-DOPA,
7-nitroindazole,
L-NAME,
Nitric oxide,
Motor behavior
Streszczenia
Język
en
Treść
Reciprocal interactions between nitrergic and dopaminergic systems play a key role in the control of motor behavior. In the present study, we performed a comparative analysis of motor behavior (locomotor activity, catalepsy, rotational behavior) and monoamine metabolism in the striatum and substantia nigra of unilaterally sham-operated and 6-OHDA-lesioned rats treated with the preferential neuronal nitric oxide synthase (nNOS) inhibitor 7-nitroindazole (7-NI) or the non-selective one N-G-nitro-L-arginine methyl ester (L-NAME), alone or in combination with L-DOPA. Each NOS inhibitor given alone (50 mg/kg) induced a distinct catalepsy 30 min after injection but only 7-NI impaired spontaneous locomotion after 10 mm. In 6-OHDA-lesioned rats, chronic L-DOPA (25 mg/kg) induced 2.5-h long contralateral rotations. 7-NI (30 and 50 mg/kg) markedly reduced the intensity of L-DOPA-induced contralateral rotations while extending their duration until 4.5 h whereas L-NAME (50 and 100 mg/kg) only tended to attenuate their intensity without affecting the duration. 7-NI but not L-NAME significantly increased endogenous tissue DA levels in the nigrostriatal system of both sham-operated and 6-OHDA-lesioned rats. In L-DOPA-treated group, 7-NI significantly enhanced the L-DOPA-derived tissue DA content in this system and decreased the level of the intracellular DA metabolite DOPAC produced by monoamine oxidase (MAO). In contrast to 7-NI, L-NAME decreased markedly DA content and did not affect DOPAC level in the ipsilateral striatum. It means that the differences in 7-NI and L-NAME-mediated modulation of L-DOPA-induced behavioral and biochemical effects resulted not only from the inhibition of NOS activity but also from differences in their ability to inhibit MAO. (C) 2015 Elsevier B.V. All rights reserved.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
697862
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych