Early diagnosis of fungal infections using piezomicrogravimetric and electric chemosensors based on polymers molecularly imprinted with D-arabitol
PBN-AR
Instytucja
Instytut Chemii Fizycznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Biosensors & Bioelectronics
ISSN
0956-5663
EISSN
1873-4235
Wydawca
ELSEVIER ADVANCED TECHNOLOGY
DOI
URL
Rok publikacji
2016
Numer zeszytu
Strony od-do
627-635
Numer tomu
79
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Molecularly imprintedpolymer(MIP)
Polybithiophene
D-arabitol
Quartz crystal resonator (QCR) chemosensor
Extended-gate field-effect transistor (EG- FET)
Candidiasis diagnosis
Streszczenia
Język
en
Treść
An elevated concentration of D-arabitol in urine, especially compared to that of L-arabitol or creatinine, is indicative of a fungal infection. For that purpose, we devised, fabricated, and tested chemical sensors determining D-arabitol. These chemosensors comprised the quartz crystal resonator (QCR) or extended-gate field-effect transistor (EG-FET) transducers integrated with molecularly imprinted polymer (MIP) film recognition units. To this end, we successfully applied a covalent approach to molecular imprinting, which involved formation of weak reversible covalent bonds between vicinal diols of arabitol and boronic acid substituents of the bithiophene functional monomer used. The MIP films were synthesized and simultaneously deposited on gold electrodes of quartz crystal resonators (Au-QCRs) or Au-glass slides by potentiodynamic electropolymerization. With the QCR and EG-FET chemosensors, the D-arabitol concentration was determined under flow-injection analysis and stagnant-solution binding conditions, respectively. Selectivity with respect to common interferences, and L-arabitol in particular, of the devised chemosensors was superior. Limits of detection (linear dynamic concentration range) of the QCR and EG-FET chemosensors were 0.15 mM (0.15 – 1.25 mM) and 0.12 mM (0.12 – 1.00 mM), respectively, and were lower than the D-arabitol concentrations in urine of patients with invasive candidiasis (>220 μM). Therefore, the devised chemosensors are suitable for early diagnosis of fungal infections caused by Candida sp. yeasts.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
689968
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