Effect Of Static Magnetic Fields And Phloretin On Antioxidant Defense System Of Human Fibroblasts
PBN-AR
Instytucja
Sieć Badawcza Łukasiewicz - Instytut Napędów i Maszyn Elektrycznych KOMEL
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
Environmental Science and Pollution Research
ISSN
0944-1344
EISSN
Wydawca
Springer
DOI
Rok publikacji
2016
Numer zeszytu
23/2016
Strony od-do
14989-14996
Numer tomu
Identyfikator DOI
Liczba arkuszy
0,5
Słowa kluczowe
angielski
Static magnetic field, QRT-PCR, Fibroblasts, Phloretin Antioxidant defense system, Redox homeostasis
Open access
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Ilość miesięcy od publikacji
1
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Streszczenia
Język
angielski
Treść
The available evidence from in vitro and in vivo studies is deemed not sufficient to draw conclusions about the potential health effects of static magnetic field (SMF) exposure. Therefore, the aim of the present study was to determine the influence of static magnetic fields and phloretin on the redox homeostasis of human dermal fibroblasts. Control fibroblasts and fibroblasts treated with phloretin were subjected to the influence of static magnetic fields. Three chambers with static magnetic fields of different intensities (0.4, 0.55, and 0.7 T) were used in the study. Quantification of superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), glutathione peroxidase 1 (GPX1), microsomal glutathione S-transferase 1 (MGST1), glutathione reductase (GSR), and catalase (CAT) messenger RNAs (mRNAs) was performed by means of real-time reverse transcription PCR (QRT-PCR) technique. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities were measured using a commercially available kit. No significant differences were found in SOD1, SOD2, GPX1, MGST1, GSR, and CAT mRNA levels among the studied groups in comparison to the control culture without phloretin and without the magnet. There were also no changes in SOD, GPx, and CAT activities. In conclusion, our study indicated that static magnetic fields generated by permanent magnets do not exert a negative influence on the oxidative status of human dermal fibroblasts. Based on these studies, it may also be concluded that phloretin does not increase its antioxidant properties under the influence of static magnetic fields. However, SMF-induced modifications at the cellular and molecular level require further clarification.
Inne
System-identifier
PX-579ff0acc2dc637166fe5e3c
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