Methods for the cultivation of ciliated protozoa from the large intestine of horses
PBN-AR
Instytucja
Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
FEMS Microbiology Letters
ISSN
0378-1097
EISSN
1574-6968
Wydawca
WILEY-BLACKWELL
DOI
URL
Rok publikacji
2016
Numer zeszytu
2
Strony od-do
brak
Numer tomu
363
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
horse
protozoa
Blepharocorys
cultivation
in vitro
Streszczenia
Język
en
Treść
This paper describes cultivation methods for ciliates from the digestive tract of horses. Members of three different genera were successfully grown in vitro for short periods of time. However, only cells belonging to the genus Blepharocorys, which resides in the horse's large intestine, were maintained for longer periods. This Blepharocorys culture was successfully grown in vitro after inoculation of freshly excreted horse faeces in culture medium containing a population of bacteria. The ciliates survived for over six months, and the density of their population varied between 1.7 × 103 and 2.4 × 103 cells mL−1. Favourable conditions for the prolonged cultivation of this ciliate were observed when the medium was prepared by mixing horse faeces and ‘caudatum’ salt solution in a 1:1 V/V ratio together with food (60% powdered meadow hay, 16% wheat gluten, 12% barley flour and 12% microcrystalline cellulose) supplied as 0.20 mg mL−1 culture per day.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
721443
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