A single amino acid substitution in movement protein of tomato torrado virus influences ToTV infectivity in Solanum lycopersicum.
PBN-AR
Instytucja
Instytut Ochrony Roślin - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
VIRUS RESEARCH
ISSN
0168-1702
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2016
Numer zeszytu
Strony od-do
32-36
Numer tomu
213
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Movement protein; Mutation; Pathogenicity determinant; Plant response; Symptom expression; Tomato torrado virus
Streszczenia
Język
en
Treść
Tomato torrado virus (ToTV), which is a tomato-infecting member of the genus Torradovirus, induces severe systemic necrosis in Solanum lycopersicum cv. Beta Lux as well as leaf malformation and chlorosis in Nicotiana benthamiana. To date, neither the tomato gene conferring resistance to the pathogen nor the ToTV-encoded necrosis determinant have been characterized. We herein revealed that the phenylalanine 210 residue in the movement protein domain encoded by ToTV RNA2 is a necrosis-inducing pathogenicity determinant during tomato infection. Using a ToTV infectious RNA2 clone, we performed site-directed mutagenesis of the phenylalanine 210 residue, confirming its importance during ToTV infection and symptom manifestation in S. lycopersicum cv. Beta Lux, but not in N. benthamiana.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
726552