One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus.
PBN-AR
Instytucja
Instytut Ochrony Roślin - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
ARCHIVES OF VIROLOGY
ISSN
0304-8608
EISSN
1432-8798
Wydawca
SPRINGER WIEN
DOI
URL
Rok publikacji
2016
Numer zeszytu
5
Strony od-do
1359-1364
Numer tomu
161
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Diagnostic protocol; Plant virus detection; RT-LAMP; Torradovirus; Virus diagnostics
Streszczenia
Język
en
Treść
Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
726694