Multiple Subcellular Localizations of Dehydrin-like Proteins in the Embryonic Axes of Common Beech (Fagus sylvatica L.) Seeds During Maturation and Dry Storage
PBN-AR
Instytucja
Instytut Dendrologii Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF PLANT GROWTH REGULATION
ISSN
0721-7595
EISSN
1435-8107
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2014
Numer zeszytu
7
Strony od-do
137-149
Numer tomu
42
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 3)
Pozostali autorzy
+ 1
Słowa kluczowe
en
Beech, Dehydrin, Seed development, Seed storage, Subcellular localization
Open access
Tryb otwartego dostępu
Otwarte czasopismo
Wersja tekstu w otwartym dostępie
Wersja opublikowana
Licencja otwartego dostępu
Creative Commons — Uznanie autorstwa
Czas opublikowania w otwartym dostępie
Przed publikacją
Data udostępnienia w sposób otwarty
Streszczenia
Język
en
Treść
The accumulation and localization of dehydrin-like proteins were analyzed in the embryonic axes of beech (Fagus sylvatica L.) seeds during maturation between the 12th and 19th week after flowering and in the dry state after 2 years of storage. Three dehydrin-like proteins were reported using an antibody specific to the K-segment, and their subcellular localization was investigated using an immunocytochemistry approach. All dehydrin-like proteins were phosphorylated, and two, with molecular masses of 26 and 44 kDa, accumulated in mature seeds. During seed maturation, dehydrin-like proteins were frequently detected inside the round or segmented nuclei, along the cell plasma membrane and associated with small vesicles. Moreover, they were found to be attached to the mitochondrial and amyloplast envelopes. These proteins were also located in the cytosol and were associated with membrane structures throughout the cytoplasm. Conversely, in the embryonic axes of stored seeds, fewer specific locations were found; dehydrin-like proteins were associated mostly with amyloplasts, and were detected to a lesser extent in the nuclei, vacuoles, and other cytoplasmic structures. Bioinformatic tools were used to predict the putative beech dehydrin sites that interact with DNA, proteins, and lipid membranes to highlight the reported subcellular locations. Here, we present and discuss the possible roles of dehydrin-like proteins in seeds with respect to their subcellular localizations.
Cechy publikacji
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Original article
Original article presents the results of original research or experiment.
Inne
System-identifier
PBN-R:609292
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych