Sugar availability modulates polyisoprenoid and phytosterol profiles in Arabidopsis thaliana hairy root culture.
PBN-AR
Instytucja
Instytut Biochemii i Biofizyki Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Biochimica et biophysica acta-Molecular and Cell Biology of Lipids
ISSN
0006-3002
EISSN
1388-1981
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2013
Numer zeszytu
2
Strony od-do
438-447
Numer tomu
1831
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 3
Słowa kluczowe
EN
Dolichol;
Phytosterol;
Dolichyl ester;
Steryl ester;
cis-prenyltransferase;
Arabidopsis thaliana
Streszczenia
Język
EN
Treść
Sugars are recognized as signaling molecules regulating the biosynthesis of secondary metabolites in plants. Here, a modulatory effect of sugars on dolichol and phytosterol profiles was noted in the hairy roots of Arabidopsis thaliana. Arabidopsis roots contain a complex dolichol mixture comprising three groups ('families') of dolichols differing in the chain-length. These dolichols, especially the longest ones are accompanied by considerable amounts of polyprenols of the same length. The spectrum of polyisoprenoid alcohols, i.e. dolichols and polyprenols, was dependent on sugar type (glucose or sucrose) and its concentration in the medium. Among the long-chain dolichols Dol/Pren-20 (dolichol or prenol molecule composed of 20 isoprene residues) and Dol/Pren-23 were the main components at 0.5% and 2% glucose, respectively. Moreover, the ratio of polyprenols versus respective dolichols was also modulated by sugar in this group of polyisoprenoids, with polyprenols dominating at 3% sucrose and dolichols at 2% glucose. Glucose concentration affected the expression level of genes encoding cis-prenyltransferases, enzymes responsible for elongation of the polyisoprenoid chain. The most abundant phytosterols of the A. thaliana roots, β-sitosterol, stigmasterol and campesterol, were accompanied by corresponding stanols and traces of brassicasterol, stigmast-4,22-dien-3-one and stigmast-4-en-3-one. Similar to the polyisoprenoids, sterol profile responded to the sugar present in the medium, β-sitosterol dominating in roots grown on 3% or lower glucose concentrations and stigmasterol in 3% sucrose. These results indicate an involvement of sugar signaling in the regulation of cis-prenyltransferases and phytosterol pathway enzymes.
Cechy publikacji
Article
Inne
System-identifier
4703
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