INFLUENCE OF CLOUDY APPLE JUICE ON N-NITROSODIETHYLAMINEINDUCED LIVER INJURY AND PHASES I AND II BIOTRANSFORMATION ENZYMES IN RAT LIVER
PBN-AR
Instytucja
Instytut Ogrodnictwa
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Acta Poloniae Pharmaceutica - Drug Research
ISSN
0001-6837
EISSN
2353-5288
Wydawca
Polskie Towarzystwo Farmaceutyczne
DOI
Rok publikacji
2015
Numer zeszytu
2
Strony od-do
267-276
Numer tomu
72
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
cloudy apple juice,
cytochrome P450,
GST,
NDEA,
NQO1,
comet assay,
Open access
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Wersja opublikowana
Licencja otwartego dostępu
Inna
Czas opublikowania w otwartym dostępie
Razem z publikacją
Data udostępnienia w sposób otwarty
Streszczenia
Język
en
Treść
Cloudy apple juice (CAJ) is a rich source of nutrients as well as non-nutrient components including high quantity of polyphenols, particularly oligomeric procyanidins, which are considered as potential chemopreventive agents that protect against the action of chemical carcinogens. The aim of this study was to examine the effect of CAJ alone or in combination with hepatocarcinogenic N-nitrosodiethylamine (NDEA) on liver damage biomarkers, including DNA damage, and the phase I and II enzymes in rat. The forced feeding with CAJ alone for 28 days, has slightly reduced the activities of phase I enzymes, MROD (CYP1A2 biomarker) and PNPH (CYP2E1 biomarker), while phase II enzymes, glutathione S-transferase (GST) and NAD(P)H:quinone oxidoreductase-1 (NQO1), were elevated. Combined treatment of rats with CAJ and NDEA significantly reduced the levels of hepatic ALT and SDH (by ~100%) as compared to values from NDEA-treated animals. CAJ pretreatment further increased the PROD (CYP2B biomarker) and NQO1 activities increased by NDEA administration. Modulation of enzymes activities was accompanied by the changes in the proteins levels. These results indicate that CAJ may protect liver against damage induced by NDEA. Moreover, a significant decrease of SDH activity by CAJ may confirm its potential anti-diabetic activity.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
608625