Lipoprotein remodeling generates lipid-poor apolipoprotein A-I particles in human interstitial fluid
PBN-AR
Instytucja
Instytut Medycyny Doświadczalnej i Klinicznej im. Mirosława Mossakowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
ISSN
0193-1849
EISSN
1522-1555
Wydawca
AMER PHYSIOLOGICAL SOC
DOI
URL
Rok publikacji
2013
Numer zeszytu
3
Strony od-do
321-E328
Numer tomu
304
Identyfikator DOI
Liczba arkuszy
1,25
Słowa kluczowe
en
apolipoprotein A-I
cholesterol
high-density lipoproteins
interstitial fluid
peripheral lymph
Streszczenia
Język
en
Treść
Miller NE, Olszewski WL, Hattori H, Miller IP, Kujiraoka T, Oka T, Iwasaki T, Nanjee MN. Lipoprotein remodeling generates lipid-poor apolipoprotein A-I particles in human interstitial fluid. Am J Physiol Endocrinol Metab 304: E321-E328, 2013. First published December 11, 2012; doi:10.1152/ajpendo.00324.2012.-Although much is known about the remodeling of high density lipoproteins (HDLs) in blood, there is no information on that in interstitial fluid, where it might have a major impact on the transport of cholesterol from cells. We incubated plasma and afferent (prenodal) peripheral lymph from 10 healthy men at 37 degrees C in vitro and followed the changes in HDL subclasses by nondenaturing two-dimensional crossed immunoelectrophoresis and size-exclusion chromatography. In plasma, there was always initially a net conversion of small pre-beta-HDLs to cholesteryl ester (CE)-rich alpha-HDLs. By contrast, in lymph, there was only net production of pre-beta-HDLs from alpha-HDLs. Endogenous cholesterol esterification rate, cholesteryl ester transfer protein (CETP) concentration, CE transfer activity, phospholipid transfer protein (PLTP) concentration, and phospholipid transfer activity in lymph averaged 5.0, 10.4, 8.2, 25.0, and 82.0\% of those in plasma, respectively (all P < 0.02). Lymph PLTP concentration, but not phospholipid transfer activity, was positively correlated with that in plasma (r = +0.63, P = 0.05). Mean PLTP-specific activity was 3.5-fold greater in lymph, reflecting a greater proportion of the high-activity form of PLTP. These findings suggest that cholesterol esterification rate and PLTP specific activity are differentially regulated in the two matrices in accordance with the requirements of reverse cholesterol transport, generating lipid-poor pre-beta-HDLs in the extracellular matrix for cholesterol uptake from neighboring cells and converting pre-beta-HDLs to alpha-HDLs in plasma for the delivery of cell-derived CEs to the liver.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
596555
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych