Crosstalk Between Autophagy and Apoptosis in RAW 264.7 Macrophages Infected With Ectromelia Orthopoxvirus
PBN-AR
Instytucja
Instytut Medycyny Doświadczalnej i Klinicznej im. Mirosława Mossakowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Viral Immunology
ISSN
0882-8245
EISSN
1557-8976
Wydawca
MARY ANN LIEBERT INC
DOI
URL
Rok publikacji
2013
Numer zeszytu
5
Strony od-do
322-335
Numer tomu
26
Identyfikator DOI
Liczba arkuszy
1,5
Słowa kluczowe
en
CASPASE-MEDIATED CLEAVAGE
PROGRAMMED CELL-DEATH
IN-VIVO
VIRUS-INFECTION
BECLIN 1
MACHINERY
PROTEINS
VITRO
REPLICATION
MECHANISM
Streszczenia
Język
en
Treść
Several studies have provided evidence that complex relationships between autophagic and apoptotic cell death pathways occur in cancer and virus-infected cells. Previously, we demonstrated that infection of macrophages with Moscow strain of ectromelia virus (ECTV-MOS) induces apoptosis under in vitro and in vivo conditions. Here, we found that autophagy was induced in RAW 264.7 cells during infection with ECTV-MOS. Silencing of beclin 1, an autophagy-related gene, reduced the percentage of late apoptotic cells in virus-infected RAW 264.7 macrophages. Pharmacological modulation of autophagy by wortmannin (inhibitor) or rapamycin (inductor) did not affect or cause increased apoptosis in ECTV-MOS-infected RAW 264.7 cells, respectively. Meantime, blocking apoptosis by a pan-caspase inhibitor, Z-VAD-FMK, increased the formation of autophagosomes in infected macrophages. Taken together, three important points arise from our study. First, autophagy may co-occur with apoptosis in RAW 264.7 cells exposed to ECTV-MOS. Second, at later stages of infection, autophagy may partially participate in the execution of macrophage cell death by enhancing apoptosis. Third, when apoptosis is blocked infected macrophages undergo increased autophagy. Our results provide new information about the relationship between autophagy and apoptosis in ECTV-MOS-infected macrophages.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
596162
CrossrefMetadata from Crossref logo
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