Comparative \emph {in vitro} study of calcium phosphate ceramics for their potency as scaffolds for tissue engineering
PBN-AR
Instytucja
Wydział Inżynierii Materiałowej i Ceramiki (Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie)
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Bio-Medical Materials and Engineering
ISSN
0959-2989
EISSN
1878-3619
Wydawca
IOS Press
Rok publikacji
2014
Numer zeszytu
3
Strony od-do
1609--1623
Numer tomu
24
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
1.07
Autorzy
Pozostali autorzy
+ 5
Słowa kluczowe
EN
ceramics
tissue engineering
osteoblast
biphasic calcium phosphate
dynamic culture
Streszczenia
Język
EN
Treść
BACKGROUND: Calcium phosphate ceramics have been widely considered as scaffolds for bone tissue engineering. Selection of the best support for cultured cells, crucial for tissue engineered systems, is still required. OBJECTIVE: We examined three types of calcium phosphate compounds: α-tricalcium phosphate-the most soluble one, carbonate hydroxyapatite-chemically the most similar to the bone mineral and biphasic calcium phosphate-with the best in vivo biocompatibility in order to select the best support for osteoblastic cells for tissue engineered systems. METHODS: Human osteoblasts were tested in direct contact with both dense samples and 3D scaffolds in either static or dynamic culture. Cell viability, cell spreading, osteogenic cell capacity, and extracellular matrix production were examined. RESULTS: The obtained data indicate that biphasic calcium phosphate is the optimal cell-supporting material. In addition, dynamic culture improved cell distribution in the scaffolds, enhanced production of the extracellular matrix and promoted cells osteogenic capacity. CONCLUSIONS: Biphasic calcium phosphate should be recommended as the most suitable matrix for osteogenic cells expansion and differentiation in tissue engineered systems. © 2014 - IOS Press and the authors.
Cechy publikacji
original article
peer-reviewed
Inne
System-identifier
idp:086801
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