A microfluidic system to study the cytotoxic effect of drugs: the combined effect of celecoxib and 5-fluorouracil on normal and cancer cells
PBN-AR
Instytucja
Wydział Chemiczny (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Microchimica Acta
ISSN
0026-3672
EISSN
Wydawca
Springer Wien
DOI
Rok publikacji
2013
Numer zeszytu
9
Strony od-do
895-901
Numer tomu
180
Identyfikator DOI
Liczba arkuszy
0.5
Autorzy
Pozostali autorzy
+ 2
Słowa kluczowe
en
chemotherapy resistance, multidrug tests, microfluidic gradient system, lab-on-a-chip, celecoxib, 5-fluorouracil
Streszczenia
Język
en
Treść
We have investigated the response of normal and cancer cells to exposure a combination of celecoxib (Celbx) and 5-fluorouracil (5-FU) using a lab-on-a-chip microfluidic device. Specifically, we have tested the cytotoxic effect of Celbx on normal mouse embryo cells (Balb/c 3T3) and human lung carcinoma cells (A549). The single drugs or their combinations were adjusted to five different concentrations using a concentration gradient generator (CGG) in a single step. The results suggest that Celbx can enhanced the anticancer activity of 5-FU by stronger inhibition of cancer cell growth. We also show that the A549 cancer cells are more sensitive to Celbx than the Balb/c 3T3 normal cells. The results obtained with the microfluidic system were compared to those obtained with a macroscale in vitro cell culture method. In our opinion, the microfluidic system represents a unique approach for an evaluation of cellular response to multidrug exposure that also is more simple than respective microwell plate assays.
Inne
System-identifier
WUT402711
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