Effect of particle size and dispersion status on cytotoxicity and genotoxicity of zinc oxide in human bronchial epithelial cells.
PBN-AR
Instytucja
Instytut Medycyny Pracy im. prof. dr. med. Jerzego Nofera
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Mutation Research. Genetic Toxicology and Environmental Mutagenesis
ISSN
1383-5718
EISSN
Wydawca
DOI
URL
Rok publikacji
2016
Numer zeszytu
Strony od-do
7-18
Numer tomu
805
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 8)
Pozostali autorzy
+ 6
Słowa kluczowe
PL
tlenek cynku
uszkodzenia DNA
genotoksyczność
mikrojądra
nanocząstki
Streszczenia
Język
EN
Treść
Data available on the genotoxicity of zinc oxide (ZnO) nanoparticles (NPs) are controversial. Here, we examined the effects of particle size and dispersion status on the cytotoxicity and genotoxicity of nanosized and fine ZnO, in the presence and absence of bovine serum albumin (BSA; 0.06%) in human bronchial epithelial BEAS-2B cells. Dynamic light scattering analysis showed the most homogenous dispersions in water alone for nanosized ZnO and in water with BSA for fine ZnO. After a 48-h treatment, both types of ZnO were cytotoxic within a similar, narrow dose range (1.5??3.0 ?g/cm2) and induced micronuclei at a near toxic dose range (1.25??1.75 ?g/cm2), both with and without BSA. In the comet assay, nanosized ZnO (1.25??1.5 ?g/cm2), in the absence of BSA, caused a statistically significant increase in DNA damage after 3-h and 6-h treatments, while fine ZnO did not. Our findings may be explained by better uptake or faster intracellular dissolution of nanosized ZnO without BSA during short treatments (3??6 h; the comet assay), with less differences between the two ZnO forms after longer treatments (>48 h; the in vitro micronucleus test). As ZnO is genotoxic within a narrow dose range partly overlapping with cytotoxic doses, small experimental differences e.g. in the dispersion of ZnO particles may have a substantial effect on the genotoxicity of the nominal doses added to the cell culture.
Inne
System-identifier
100 408 526
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