Endurance training upregulates the nitric oxide/soluble guanylyl cyclase/cyclic guanosine 3 `,5 `-monophosphate pathway in the striatum, midbrain and cerebellum of male rats
PBN-AR
Instytucja
Instytut Medycyny Doświadczalnej i Klinicznej im. Mirosława Mossakowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Brain Research
ISSN
0006-8993
EISSN
1872-6240
Wydawca
ELSEVIER SCIENCE BV
Rok publikacji
2015
Numer zeszytu
Strony od-do
29-40
Numer tomu
1618
Identyfikator DOI
Liczba arkuszy
1,41
Słowa kluczowe
en
Brain-derived neurotrophic factor
Endurance training
Soluble guanylyl cyclase
Locomotion
Nitric oxide synthase
Streszczenia
Język
en
Treść
The nitric oxide/soluble guanylyl cyclase/cyclic guanosine monophosphate (NO/sGC/cGMP) brain pathway plays an important role in motor control. We studied the effects of 6-week endurance training (running) of moderate intensity on this pathway by comparing, between sedentary and endurance-trained young adult male Wistar rats, the expression of endothelial (eNOS) and neuronal (nNOS) NO synthases and of alpha 1, alpha 2 and beta 1. GC subunits, as well as cGMP levels, in the brain cortex, hippocampus, striatum, midbrain and cerebellum. Additionally, we compared the respective regional expressions of BDNF and the BDNF receptor TrkB. Twenty-four hours after the last training session, the endurance-trained rats showed 3-fold higher spontaneous locomotor activity than their sedentary counterparts in an open-field test. Forty-eight hours after the completion of the training, the trained rats showed significantly elevated BDNF and TrKB mRNAs in the hippocampus, midbrain and striatum, and significantly increased BDNF levels in the hippocampus and striatum. Simultaneously, significant increases were found in mRNA and protein levels and activities of nNOS and eNOS as well as in mRNA and protein levels of GC alpha 2 and GC beta 1, but not GC alpha 1, in the striatum, midbrain and cerebellum; no change in these variables was found in the cortex and hippocampus except for marked elevations in cortical GC beta 1 mRNA and protein. Changes in regional cGMP levels paralleled those in eNOS, nNOS and GC alpha 2 expression and NOSs' activities. These results suggest that favorable extrapyramidal motor effects of physical training are related to the enhanced activity of the NO/sGC/cGMP pathway in certain motor control-related subcortical brain regions. (C) 2015 Elsevier B.V. All rights reserved.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
626799
CrossrefMetadata from Crossref logo
Cytowania
Liczba prac cytujących tę pracę
Brak danych
Referencje
Liczba prac cytowanych przez tę pracę
Brak danych