Baclofen or nNOS inhibitor affect molecular and behavioral alterations evoked by traumatic spinal cord injury in rat spinal cord
PBN-AR
Instytucja
Instytut Medycyny Doświadczalnej i Klinicznej im. Mirosława Mossakowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Spine Journal
ISSN
1529-9430
EISSN
Wydawca
ELSEVIER SCIENCE INC
DOI
URL
Rok publikacji
2015
Numer zeszytu
6
Strony od-do
1366-1378
Numer tomu
15
Identyfikator DOI
Liczba arkuszy
1,79
Słowa kluczowe
en
Baclofen
NNLA
Spinal cord injury
alpha-Motoneurons
Tail-flick text
Reflex response
Neuronal nitric oxide synthase
Rat
Streszczenia
Język
en
Treść
BACKGROUND CONTEXT: The loss of descending control after spinal cord injury (SCI) and incessant stimulation of Ia monosynaptic pathway, carrying proprioceptive impulses from the muscles and tendons into the spinal cord, evoke exaggerated alpha-motoneuron activity leading to increased reflex response. Previous results from our laboratory have shown that Ia monosynaptic pathway is nitrergic. PURPOSE: The aim of this study was to find out whether nitric oxide produced by neuronal nitric oxide synthase (nNOS) plays a role in setting the excitability of alpha-motoneurons after thoracic spinal cord transection. STUDY DESIGN: We tested the hypothesis that the inhibition of nNOS in alpha-motoneurons after SCI could have a neuroprotective effect on reflex response. METHODS: Rats underwent spinal cord transection at Th10 level followed by 7, 10, and 14 days of survival. The animals were treated with Baclofen (a gamma aminobutyric acid B receptor agonist, 3 mu g/two times per day/intrathecally) applied for 3 days from the seventh day after transection; N-nitro-L-arginine (NNLA) (nNOS blocator) applied for the first 3 days after injury (20 mg/kg per day, intramuscularly); NNLA and Baclofen; or NNLA (60 mg/kg/day, single dose) applied on the 10th day after transection. We detected the changes in the level of nNOS protein, nNOS messenger RNA, and nNOS immunoreactivity. To investigate the reflex response to heat-induced stimulus, tail-flick test was monitored in treated animals up to 16 days after SCI. RESULTS: Our data indicate that Baclofen therapy is more effective than the combined treatment with NNLA and Baclofen therapy. The single dose of NNLA (60 mg/kg) applied on the 10th day after SCI or Baclofen therapy reduced nNOS expression in a-motoneurons and suppressed symptoms of increased reflex activity. CONCLUSIONS: The results clearly show that increased nNOS expression in a-motoneurons after SCI may be pharmacologically modifiable with Baclofen or bolus dose of nNOS blocker. (C) 2015 Published by Elsevier Inc.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
626791
CrossrefMetadata from Crossref logo
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