Doxycycline depletion and residues in eggs after oral administration to laying hens
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Food Additives & Contaminants: Part A
ISSN
1944-0049
EISSN
1944-0057
Wydawca
Abingdon, Oxon ; Philadelphia, PA : Taylor & Francis
DOI
URL
Rok publikacji
2015
Numer zeszytu
7
Strony od-do
1116-1123
Numer tomu
32
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 2)
Słowa kluczowe
en
doxycycline
depletion
residues
eggs
LC-MS/MS
Streszczenia
Język
en
Treść
The depletion of doxycycline (DC) residues in eggs was determined after oral drug administration by drinking water to laying hens. The antibiotic was supplied to birds for 5 consecutive days and the eggs were collected during medication and 18 days after withdrawal. DC residues were determined by LC-MS/MS. DC was isolated from eggs with a solution of 0.02 M of oxalic acid (pH 4), 0.1 M Na2EDTA and acetonitrile. The limit of detection (LOD) and limit of quantification (LOQ) of the method were 2 and 5 µg kg–1, respectively. Analyses were performed on whole egg, egg white and yolk separately. DC was detectable 24 h after the beginning of administration. The concentration of antibiotic increased daily, resulting in the highest DC concentration in whole eggs at the first day of the withdrawal period. Thirteen days after withdrawal, the content of DC in whole eggs was below the LOQ of the method. However, some differences were found in the depletion curve of DC between egg white and yolk. Residues of DC in egg white were much higher during treatment and 1 day after withdrawal, but later the concentration in egg white decreased fairly rapidly and a higher DC content in egg yolk was observed. The depletion period was shorter for egg white than for yolk, and DC was detected in the egg white until 12 days after withdrawal and 2 days more in egg yolk than in white. DC reached a peak faster in egg white, but the residues were detectable for longer period in the yolk.
Cechy publikacji
praca doświadczalna
Inne
System-identifier
PX-56b4596a8106eb71826de82b
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