Ocena skuteczności metod inwersji faz antygenów rzęskowych Salmonella
PBN-AR
Instytucja
Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
pl
Czasopismo
Medycyna Weterynaryjna
ISSN
0025-8628
EISSN
Wydawca
POLISH SOC VETERINARY SCIENCES EDITORIAL OFFICE
DOI
Rok publikacji
2015
Numer zeszytu
12
Strony od-do
796-800
Numer tomu
71
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
pl
Salmonella
phase inversion
flagellar antigens
Open access
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Creative Commons — Uznanie autorstwa-Na tych samych warunkach
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Razem z publikacją
Streszczenia
Język
pl
Treść
Expression of flagellar antigens is necessary to determine the complete antigenic structure of a Salmonella isolate. In many cases, this involves techniques that make it possible to select bacterial cells capable of expressing the currently inhibited antigen phase. The aim of the study was to compare the effectiveness of three available methods used for phase inversion of the flagellar antigens of Salmonella: Gard’s plate, Craigie’s tube and the paper bridge method. A total of 137 isolates with the expression of somatic antigen and only one of the two flagella phases were selected for the study. The three techniques identified Salmonella serovars in 83.2% (114/137) of the isolates tested. The paper bridge method proved to be the most effective technique, identifying inhibited antigens in 97.3% of isolates, followed by Gard’s plate and Craigie’s tube, which were successful in, respectively, 60.5% and 40.3% of cases. The specificity of each method was 100%. The test lasted 24 hours for all isolates tested by Gard’s plate method, from 48 to 96h in the paper bridge method, and at least 48 h in Craigie’s tube method. The results show that the paper bridge method is a simple and inexpensive technique of phase inversion, highly effective in Salmonella identification. However, if rapid identification is required, Gard’s plate can be used simultaneously with the paper bridge method, so that the test can be continued by the latter method if the expected result is not achieved within the first day.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
704845