Antioxidative effects of extracts from Trifolium species on blood platelets exposed to oxidative stress
PBN-AR
Instytucja
Instytut Uprawy Nawożenia i Gleboznawstwa - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
ISSN
1138-7548
EISSN
1877-8755
Wydawca
SERVICIO PUBLICACIONES UNIVERSIDAD NAVARRA
DOI
URL
Rok publikacji
2013
Numer zeszytu
4
Strony od-do
879-887
Numer tomu
69
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Trifolium
Clover
Antioxidant
Platelets
Oxidative stress
Streszczenia
Język
en
Treść
Clovers (Trifolium) may possess a significant therapeutic potential, but the effects of compounds from these plants on blood platelets and haemostasis have been poorly recognized. The present study was designed to evaluate the antioxidative action of extracts from three species of clovers: Trifolium pratense, Trifolium pallidum and Trifolium scabrum in the protection of human blood platelets in vitro. Platelet suspensions were pre-incubated with crude extract and phenolic fraction of T. pratense or phenolic fractions of T. scabrum and T. pallidum, at the final concentrations of 0.5–50 μg/ml. Then, for the induction of oxidative stress, 100 μM peroxynitrite was added. The antioxidative activity of plant extracts was assessed by measurements of the level of 3-nitrotyrosine, thiol groups and lipid peroxidation products (hydroperoxides and thiobarbituric acid-reactive substances). Despite the significant differences in the composition of the investigated extracts, we observed antioxidative effects of all used mixtures. The presence of Trifolium extracts considerably reduced the peroxynitrite-mediated modifications of proteins and diminished peroxidation of lipids in platelets. Our results indicate on a strong antioxidative activity of the tested extracts-statistically significant effects were found even for the lowest concentrations (0.5 μg/ml) of all extracts. This action may be useful in the protection of blood components, very susceptible to oxidative modifications. The obtained results suggest that the examined clovers are a promising source of compounds, valuable for the protection against oxidative stress-induced damage to blood platelets
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
570091
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