Mutagenicity of organic pollutants adsorbed on suspended particulate matter in the center of Wrocław (Poland)
PBN-AR
Instytucja
Wydział Inżynierii Środowiska (Politechnika Wrocławska)
Informacje podstawowe
Główny język publikacji
eng
Czasopismo
ATMOSPHERIC ENVIRONMENT
ISSN
1352-2310
EISSN
Wydawca
DOI
URL
Rok publikacji
2014
Numer zeszytu
Strony od-do
620-628
Numer tomu
vol. 95
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
pol
zanieczyszczenie powietrza
mutagenność
współczynnik mutagenności
frakcja PM10
test Salmonella
Streszczenia
Język
eng
Treść
Mutagenicity of pollutants adsorbed on suspended dust of the PM10 fraction, collected in winter and summer season alike over the Wrocław city centre (Poland) was studied using the standard Salmonella assay (plate-incorporation) and the Kado modified assay (microsuspension method). The dust was collected using Staplex high volume air sampler. Further on it was extracted with dichloromethane in a Soxhlet apparatus. PAH content in extracts was determined by the high performance liquid chromatography technique using fluorescence detection, whereas the nitro-PAH content– by the gas chromatography using mass detection. Two Salmonella typhimurium strains, TA98 and YG1041, were used in the assays. The assays were conducted with and without a metabolic activation. Investigated air pollution extracts differed against each other with regard to a total content as well as to a percentage of individual compounds, depending on the sampling season. Both the total PAH content and the nitro-PAH content in the tested samples, and their spectrum as well, were found the highest in winter season. Higher mutagenic effect was noted for the dust extract from samples collected in wintertime than from those collected in summer. Pollutants directly affecting the genetic material and those showing such indirect action were present in the examined samples. The YG1041 strain turned out to be the most sensitive, which was the sign that large amounts of nitro-aromatic compounds were present in the tested samples. Obtained results proved that the Kado modified Salmonella assay would be useful for the atmospheric air pollution monitoring in urban agglomerations. Mutagenic effect in assays conducted according to the Kado procedure was obtained by using in the assays lower concentrations of tested extracts, compared to the classical assay.
Inne
System-identifier
000193033
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