Application of quartz tuning forks for detection of endotoxins and Gram-negative bacterial cells by monitoring of Limulus Amebocyte Lysate coagulation
PBN-AR
Instytucja
Wydział Elektroniki Mikrosystemów i Fotoniki (Politechnika Wrocławska)
Informacje podstawowe
Główny język publikacji
eng
Czasopismo
BIOSENSORS & BIOELECTRONICS
ISSN
0956-5663
EISSN
Wydawca
DOI
URL
Rok publikacji
2014
Numer zeszytu
Strony od-do
132-137
Numer tomu
vol. 58
Identyfikator DOI
Liczba arkuszy
Autorzy
Pozostali autorzy
+ 3
Słowa kluczowe
pol
LAL
endotoksyny
kamerton kwarcowy
pomiar lepkości
Streszczenia
Język
eng
Treść
Endotoxins, pyrogens of bacterial origin, are a significant threat in many areas of life. Currently, the test most commonly used for endotoxin level determination is LAL (Limulus Amebocyte Lysate) assay. This paper presents application of commercially available low-frequency piezoelectric tuning forks (QTFs) for endotoxin detection. Measurement of the decrease in the QTF oscillation amplitude provides information about the viscosity changes, occurring in the tested sample upon addition of LAL. That method was used to determine the concentrations of endotoxins and bacterial cells (E. coli O157:H19). The relevance of the obtained results was confirmed using a commercially available colorimetric LAL assay. The constructed system can detect bacterial endotoxins in the range of 0.001–5 EU/ml and bacterial cells in the range of 102–107 CFU/ml. The presented technique requires very simple sample preparation and the sensor response is obtained using compact, portable readout electronics. The single test cost is low compared to commercial endotoxin assays and other novel systems based on micromechanical sensors.
Inne
System-identifier
000192663
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