Expression of MexAB-OprM efflux pump system and susceptibility to antibiotics of different Pseudomonas aeruginosa clones isolated from patients hospitalized in two intensive care units at University Hospital in Bialystok (northeastern Poland) between January 2002 and December 2009
PBN-AR
Instytucja
Wydział Biologiczno-Chemiczny (Uniwersytet w Białymstoku)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
APMIS
ISSN
0903-4641
EISSN
Wydawca
DOI
URL
Rok publikacji
2014
Numer zeszytu
2
Strony od-do
931-940
Numer tomu
122
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
1,38
Słowa kluczowe
angielski
Pseudomonas aeruginosa;
MexAB-OprM expression;
clones;
susceptibility to antibiotics
Streszczenia
Język
angielski
Treść
We investigated the genetic similarities and expression of the MexAB-OprM efflux pump system in different clones of multiresistant Pseudomonas aeruginosa strains collected from 2002 to 2009 at two intensive care units (ICU). Regula- tory and structural genes mexB, mexR, and mexA were found in 99%, 98%, and 94% of tested strains, respectively. The presence of class 1 integron was found in 90% of the strains, while class 2 integron in only one strain (Psa506). Class 3 integron was not found in any of the tested strains. Among the eleven clones identified, only two clones, I and D, exhibited higher levels of mexB gene expression than the other clones. Clone I had the highest expression (FC =10.36, p <0.05). The results of our study indicated a high level of MexAB-OprM pump expression in groups of strains isolated in the years 2008–2009 (FC =12.92, p <0.03) and 2002–2006 (FC =5.14, p <0.03). There were no sta- tistically significant differences in resistance to all tested antibiotics among the various clones. The high level of antimi- crobial resistance may have been due to the coexistence of different resistance mechanisms among the studied P. aeruginosa strains. However, this does not exclude the contribution of the MexAB-OprM pump, particularly in resistance to meropenem and ciprofloxacin.
Cechy publikacji
publikacja naukowa oryginalna
Inne
System-identifier
000036374
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