The effect of intracerebroventricular infusions of ghrelin on the secretory activity of the GnRH/LH system in peripubertal female sheep
PBN-AR
Instytucja
Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Animal and Feed Sciences
ISSN
1230-1388
EISSN
Wydawca
THE KIELANOWSKI INSTITUTE OF ANIMAL PHYSIOLOGY AND NUTRITION
DOI
Rok publikacji
2014
Numer zeszytu
4
Strony od-do
299-308
Numer tomu
23
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
hypothalamus
pituitary
GnRH
LH
ghrelin
peripubertal ewes
Streszczenia
Język
en
Treść
Ghrelin is a 28-amino acid orexigenic peptide, synthesized mainly by cells located in the stomach mucosa, that can be also engaged in modula- tion of reproductive functions. The aim of this study was to investigate the ef- fect of intracerebroventricular (icv) infusion of ghrelin on the secretory activity of the gonadotropin hormone releasing hormone/luteinizing hormone (GnRH/ LH) system in peripubertal ewes. The animals were randomly divided into three groups: 1. standard fed, 2. starved for 72 h, and 3. standard fed + icv infused with ghrelin. Infusions were performed during three consecutive days; blood samples were collected on day 0 and day 3. Ghrelin infusions and short-term starvation increased the accumulation of immunoreactive (ir) GnRH material in the median eminence (P < 0.001) and ir LH material in the secretory granules of pituitary cells (P < 0.001) in the fasted and ghrelin-infused groups. The short starvation resulted in a decrease of mean LH plasma concentrations (P < 0.05) between days 0 and 3 of infusion, which was related to a lower pulse amplitude. After the icv ghrelin infusion, significant (P < 0.05) changes in LH pulse ampli- tude and frequency, but not in LH concentrations were observed. The obtained results revealed that in peripubertal ewes, exogenous ghrelin administration affects the GnRH/LH axis mainly at the level of neurohormone/protein accu- mulation and LH pulsatility modifications. Ghrelin action at the hypothalamus- pituitary axis is likely involved in the endocrine network that integrates energy balance and fertility in immature sheep.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
626628
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