Incorporation of endogenous urea nitrogen into the amino acids in bacterial protein in rumen of goats fed diets with various protein levels
PBN-AR
Instytucja
Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Animal and Feed Sciences
ISSN
1230-1388
EISSN
Wydawca
THE KIELANOWSKI INSTITUTE OF ANIMAL PHYSIOLOGY AND NUTRITION
DOI
Rok publikacji
2013
Numer zeszytu
4
Strony od-do
311-315
Numer tomu
22
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
endogenous urea
15N
rumen bacteria
protein level
dairy goats
Streszczenia
Język
en
Treść
ABSTRACT. The aim of the study was to investigate the effect of different levels of protein in a diet on the incorporation of endogenous urea nitrogen (EUN) into individual amino acids (AA) of the ruminal bacteria of goats fed a low- (LP), medium- (MP), or high-protein diet (HP) in a 3 × 3 Latin square design. Three Alpine goats of about 35 kg body weight fitted with cannula into the rumen and catheter into the jugular vein were fed three isoenergetic diets containing 11% (LP), 13% (MP), or 16% (HP) crude protein in dry matter. The goats were infused for 6 days continuously with an 15N urea solution into the jugular vein. Ruminal bacteria were hydrolysed with 6M HCl. Next, butyl derivatives of free bacterial AA were obtained using HCl in butanol, then N-acylated using trifluoroacetic acid anhydride and analysed by gas chromatography using a mass-selective detector. The concentration of urea in plasma was 178, 356 and 667 mg · l–1 in goats from groups LP, MP and HP, respectively. 15N-excess during the infusion of labelled urea was significantly higher (P < 0.05) in the vast majority of AA of ruminal bacteria from goats fed the LP diet in comparison with goats fed the HP diet. Therefore, the level of protein in the diets affects the incorporation of EUN into bacterial AA. With the LP diet, EUN was incorporated mostly into glutamic acid, isoleucine and arginine, while in the case of the HP diet, into glutamic acid and arginine, as well as methionine. Regardless of the level of nitrogen in the diets, the incorporation of 15N into proline was very low. Irrespective of the dietary nitrogen level, EUN appears to be predominantly used for synthesis of glutamic acid in ruminal bacteria.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
626527
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