Age-dependent effect of obestatin on intestinal contractility in Wistar rats
PBN-AR
Instytucja
Instytut Fizjologii i Żywienia Zwierząt im. Jana Kielanowskiego Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
General and Comparative Endocrinology
ISSN
0016-6480
EISSN
1095-6840
Wydawca
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI
URL
Rok publikacji
2014
Numer zeszytu
Strony od-do
109-115
Numer tomu
208
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Obestatin; Intestine; Contractility; Neonates
Streszczenia
Język
en
Treść
Obestatin is a 23-amino acid peptide encoded by the ghrelin gene. We have investigated the effect of obestatin on intestinal contractility in rats ranging from the suckling period till adolescence. Duodenal and middle jejunum whole-thickness preparations from neonatal and adult rats were studied in an organ bath, for isometric recording under treatment with obestatin (1 μmol L−1) in the presence of acetylocholine (ACh), atropine and tetradotoxin (TTX). Both the EFS and ACh-stimulated contractile response, as well as spontaneous contractile activity is age-dependent and specific for the segment of jejunum. Except for the middle jejunum of 7 day old rats, treatment with obestatin caused a significant TTX-sensitive increase in the amplitude of EFS-stimulated off-contraction of both intestinal segments studied. Following injection of obestatin, the amplitude of spontaneous contraction in the duodenum increased in 7 day old rats. In the middle jejunum, treatment with obestatin significantly increased both the amplitude and frequency of spontaneous contraction in rats till the 28th day of life, whereas in adult rats the observed effect of obestatin was the opposite (P < 0.001 and P < 0.0001, respectively). The effects of treatment with obestatin on stimulation with increasing doses of ACh were only observed in the preparations from suckling rats. ACh-stimulated contractility in the duodenum was decreased while in the middle jejunum the observed effect was opposite. These results indicate the importance of peripheral obestatin in the cholinergic control of intestinal contractility in both neonatal and adult rats.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
615272
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