Chemical modification of polyvinyl chloride and silicone elastomer in inhibiting adhesion of Aeromonas hydrophila
PBN-AR
Instytucja
Centrum Badań Molekularnych i Makromolekularnych Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN
0959-3993
EISSN
1573-0972
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2013
Numer zeszytu
7
Strony od-do
1197-1206
Numer tomu
29
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Aeromonas hydrophila
Biofilm
Polyvinyl chloride
Silicone elastomer
Organo-silanes
Streszczenia
Język
en
Treść
Disease-causing bacteria of the genus Aeromonas are able to adhere to pipe materials, colonizing the surfaces and forming biofilms in water distribution systems. The aim of our research was to study how the modification of materials used commonly in the water industry can reduce bacterial cell attachment. Polyvinyl chloride and silicone elastomer surfaces were activated and modified with reactive organo-silanes by coupling or cocrosslinking silanes with the native material. Both the native and modified surfaces were tested using the acterial strain Aeromonas hydrophila, which was isolated from the Polish water distribution system. The surface tension of both the native and modified surfaces was measured. To determine cell viability and bacterial adhesion two methods were used, namely plate count and luminometry. Results were expressed in colony-forming units c.f.u.) and in relative light units (RLU) per cm2. Almost all the chemically modified surfaces exhibited higher anti-adhesive and anti-microbial properties in comparison to the native surfaces. Among the modifying agents examined, poly [dimethylsiloxane-co-(N,N-dimethyl-N-n-octylammoniopropyl chloride) methylsiloxane)] terminated with ydroxydimethylsilyl groups (20 %) in silicone elastomer gave the most desirable results. The surface tension of this modifier, was comparable to the non-polar native surface. However, almost half of this value was due to the result of polar forces. In this case, in an adhesion analysis, only 1 RLU cm-2 and less than 1 c.f.u. cm-2 were noted. For the native gumosil, the results were 9,375 RLU cm-2 and 2.5 9 108 c.f.u. cm-2, respectively. The antibacterial activity of active organo-silanes was associated only with the carrier surface because no antibacterial compounds were detected in liquid culture media, in concentrations that were able to inhibit cell growth.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
459691
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