Highly immunogenic prime-boost DNA vaccination protects chickens against challenge with homologous and heterologous H5N1 virus
PBN-AR
Instytucja
Sieć Badawcza Łukasiewicz - Instytut Biotechnologii i Antybiotyków
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Trials in Vaccinology
ISSN
1879-4378
EISSN
Wydawca
Elsevier B.V.
DOI
Rok publikacji
2014
Numer zeszytu
1
Strony od-do
40-46
Numer tomu
3
Identyfikator DOI
Liczba arkuszy
0,5
Słowa kluczowe
angielski
Avian influenza
H5N1
Chicken
Poultry immunization
DNA vaccine
Open access
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Licencja otwartego dostępu
Creative Commons — Uznanie autorstwa
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Razem z publikacją
Streszczenia
Język
angielski
Treść
Highly pathogenic avian influenza viruses (HPAIVs) cause huge economic losses in the poultry industry because of high mortality rate in infected flocks and trade restrictions. Protective antibodies, directed mainly against hemagglutinin (HA), are the primary means of protection against influenza outbreaks. A recombinant DNA vaccine based on the sequence of H5 HA from the H5N1/A/swan/Poland/305-135V08/2006 strain of HPAIV was prepared. Sequence manipulation included deletion of the proteolytic cleavage site to improve protein stability, codon usage optimization to improve translation and stability of RNA in host cells, and cloning into a commercially available vector to enable expression in animal cells. Naked plasmid DNA was complexed with a liposomal carrier and the immunization followed the prime–boost strategy. The immunogenic potential of the DNA vaccine was first proved in broilers in near-to-field conditions resembling a commercial farm. Next, the protective activity of the vaccine was confirmed in SPF layer-type chickens. Experimental infections (challenge experiments) indicated that 100% of vaccinated chickens were protected against H5N1 of the same clade and that 70% of them were protected against H5N1 influenza virus of a different clade. Moreover, the DNA vaccine significantly limited (or even eliminated) transmission of the virus to contact control chickens. Two intramuscular doses of DNA vaccine encoding H5 HA induced a strong protective response in immunized chicken. The effective protection lasted for a minimum 8 weeks after the second dose of the vaccine and was not limited to the homologous H5N1 virus. In addition, the vaccine reduced shedding of the virus.
Inne
System-identifier
ICIL2052110
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