Secretion of SerpinB2 from endothelial cells activated with inflammatory stimuli
PBN-AR
Instytucja
Instytut Biologii Medycznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Experimental Cell Research
ISSN
0014-4827
EISSN
1090-2422
Wydawca
ELSEVIER INC
DOI
URL
Rok publikacji
2013
Numer zeszytu
8
Strony od-do
1213-1219
Numer tomu
319
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Autorzy
Pozostali autorzy
+ 1
Słowa kluczowe
en
Double immunogold labeling; Endothelial cells; Inflammatory stimuli; Secretion; SerpinB2
Streszczenia
Język
en
Treść
Due to the lack of an N-terminal signal peptide, SerpinB2 (plasminogen activator inhibitor type 2) accumulates in cells and only a small percentage of it is secreted. The extracellular concentration of SerpinB2 significantly increases during inflammation. In the present study we investigated the mechanism with which SerpinB2 can be secreted from endothelial cells activated with LPS. We evaluated the intracellular distribution of SerpinB2 by double immunogold labeling followed by a high resolution electron microscopy analysis. We found that SerpinB2 gathers in the vesicular structures and in the endothelial cell periphery. These vesicles stained positive for the trans-Golgi network marker TGN46, which is consistent with their formation by the endoplasmatic reticulum (ER) and Golgi-dependent pathways. SerpinB2 was delivered to the plasma membrane, apparently together with TGN46 in the same vesicles, which after fusion with the membranes released cargo. Secretion of SerpinB2 was partially inhibited by brefeldin A. The secreted SerpinB2 was predominantly in its nonglycosylated 43. kDa form as evaluated by Western immunoblotting. Our data suggest that increased expression of SerpinB2 by an inflammatory stimulus is sufficient to generate structures that resemble secretory vesicles. These vesicles may represent the mechanism by which high local concentrations of SerpinB2 are released at inflammation sites from endothelial cells. © 2013 Elsevier Inc.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
603228
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