Deciphering the role of NADPH oxidase in complex interactions between maize (Zea mays L.) genotypes and cereal aphids
PBN-AR
Instytucja
Wydział Przyrodniczy (Uniwersytet Przyrodniczo-Humanistyczny w Siedlcach)
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Biochemical and Biophysical Research Communications
ISSN
0006-291X
EISSN
1090-2104
Wydawca
Elsevier
DOI
Rok publikacji
2016
Numer zeszytu
2
Strony od-do
90-95
Numer tomu
476
Identyfikator DOI
Liczba arkuszy
0,3
Autorzy
(liczba autorów: 1)
Słowa kluczowe
PL
Aktywność enzymatyczna,
Ekspresja genów,
Enzymy,
Interakcje,
Kukurydza,
Mszyca czeremchowo-zbożowa,
Mszyca zbożowa,
Oksydaza NADPH
Streszczenia
Język
EN
Treść
Plant NADPH oxidases (NOXs) encompass a group of membrane-bound enzymes participating in formation of reactive oxygen species (ROS) under physiological conditions as well as in response to environmental stressors. The purpose of the survey was to unveil the role of NADPH oxidase in pro-oxidative responses of maize (Zea mays L.) seedling leaves exposed to cereal aphids’ infestation. The impact of apteral females of bird cherry-oat aphid (Rhopalosiphum padi L.) and grain aphid (Sitobion avenae F.) feeding on expression levels of all four NADPH oxidase genes (rbohA, rbohB, rbohC, rbohD) and total activity of NOX enzyme in maize plants were investigated. In addition, inhibitory effect of diphenylene iodonium (DPI) pre-treatment on NOX activity and hydrogen peroxide content in aphid-stressed maize seedlings was studied. Leaf infestation biotests were accomplished on 14-day-old seedlings representing two aphid-resistant varieties (Ambrozja and Waza) and two aphid-susceptible ones (Tasty Sweet and Złota Karłowa). Insects’ attack led to profound upregulation of rbohA and rbohD genes in tested host plants, lower elevations were noted in level of rbohB mRNA, whereas abundance of rbohC transcript was not significantly altered. It was uncovered aphid-induced enhancement of NOX activity in examined plants. Higher increases in expression of all investigated rboh genes and activity of NADPH oxidase occurred in tissues of more resistant maize cultivars than in susceptible ones. Furthermore, DPI treatment resulted in strong reduction of NOX activity and H2O2 accumulation in aphid-infested Z. mays plants, thus evidencing circumstantial role of the enzyme in insect-elicited ROS generation.
Cechy publikacji
oryginalny artykuł naukowy
Inne
System-identifier
0272701475938
CrossrefMetadata from Crossref logo
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