Assessment of changes in sperm cell membrane integrity occurring during the storage of semen from genetically different males using two diagnostic methods
PBN-AR
Instytucja
Wydział Przyrodniczy (Uniwersytet Przyrodniczo-Humanistyczny w Siedlcach)
Informacje podstawowe
Główny język publikacji
EN
Czasopismo
Canadian Journal of Animal Science
ISSN
0008-3984
EISSN
1918-1825
Wydawca
Ottawa, Ont.: Agricultural Institute of Canada.
DOI
Rok publikacji
2014
Numer zeszytu
4
Strony od-do
601-606
Numer tomu
94
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0,3
Autorzy
(liczba autorów: 2)
Słowa kluczowe
PL
Błona komórkowa
Ejakulat
Knury
Nasienie
Plemniki
Trzoda chlewna
Streszczenia
Język
EN
Treść
The present study was carried out to assess changes in sperm cell membrane integrity occurring during the storage of semen collected from genetically different domestic male pigs. The study was aimed at assessing differences in the course of changes in the integrity of cell membranes in spermatozoa produced by males with different degrees of genetic diversity (pure-bred males, two-breed hybrids and multi-breed crosses) and testing the usefulness of two methods of sperm cell membrane integrity evaluation, based on material collected from genetically different males. The experiments were conducted on 56 ejaculates collected from 28 domestic male pigs. The examination of sperm cell membrane integrity was performed three times for each ejaculate, i.e., after 1 h, after 24 h and after 48 h from collection. The preparations for analysing cell membrane integrity were made using two methods: the SYBR 14/PI method and the eosin–nigrosin method. It was found that both SYBR 14/PI and eosin–nigrosin staining methods make it possible to successfully assess the integrity of the plasma membrane of domestic pig sperm cells under in vitro conditions. Hybrid pig spermatozoa, especially those from multi-breed crosses, better retain the integrity of their plasmalemmas than the spermatozoa of pure-bred boars. The ejaculates of Hypor cross-breed boars assessed after 1, 24 and 48 h of storage contain more spermatozoa with intact cell membranes than the ejaculates of pure-bred Duroc and Pietrain boars. The ejaculates of Hypor boars also show fewer decaying spermatozoa than those produced by pure-bred boars.
Cechy publikacji
oryginalny artykuł naukowy
Inne
System-identifier
0272601409782
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