Distribution of cytomegalovirus gN variants and associated clinical sequelae in infants
PBN-AR
Instytucja
Instytut Biologii Medycznej Polskiej Akademii Nauk
Informacje podstawowe
Główny język publikacji
en
Czasopismo
JOURNAL OF CLINICAL VIROLOGY
ISSN
1386-6532
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2013
Numer zeszytu
1
Strony od-do
271-275
Numer tomu
58
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Human cytomegalovirus; Glycoprotein N; Congenital infection
Streszczenia
Język
en
Treść
Background: Human cytomegalovirus (HCMV) is the most widespread cause of congenital infection. The effects of various viral strains and viral loads on the infection outcome have been under debate. Objectives: To determine the distribution of gN variants in HCMV strains isolated from children with congenital or postnatal infection and to establish the relationship between the viral genotype, the viral load, and the sequelae. Study design: The study population included congenitally HCMV-infected newborns and children with postnatal or unproven congenital HCMV infection. The genotyping was performed by RFLP analysis of PCR-amplified fragments, and the viral load was measured by quantitative real-time PCR. Results: Our results demonstrated that the HCMV genotypes gN3b, gN4b, and gN4c were prevalent in the patients examined. There were no differences in the distributions of gN genotypes in the congenitally and postnatally infected children. Multiple HCMV strains were detected in both groups of children. A significant association between the HCMV gN4 genotype and the incidence of neurological disorders was observed (p = 0.045). Our results suggest that the detection of the gN2 or the gN4 genotype may be indicative of serious manifestations in children. In contrast, the gN3b and the gN1 genotypes represent less pathogenic HCMV strains. The HCMV load in urine was significantly higher in children with congenital infection compared with children with postnatal infection. No correlation was found between the viral load and the genotype. Conclusion: Our results suggest that the gN genotype may be a virological marker of symptomatic HCMV infection in newborns.
Cechy publikacji
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Inne
System-identifier
634276
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