Determination of cathepsin G in endometrial tissue using a surface plasmon resonance imaging biosensor with tailored phosphonic inhibitor
PBN-AR
Instytucja
Wydział Biologiczno-Chemiczny (Uniwersytet w Białymstoku)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
European Journal of Obstetrics & Gynecology and Reproductive Biology
ISSN
0301-2115
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2014
Numer zeszytu
Strony od-do
38-42
Numer tomu
182
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0,7
Słowa kluczowe
angielski
Biosensor
Cathepsin G inhibitor
Endometrium tissue
Surface plasmon resonance imaging
Streszczenia
Język
angielski
Treść
OBJECTIVE: Cathepsin G is a serine peptidase whose physiological role is mainly associated with an early immune response, anti-microbial activity as well as platelet activation or hydrolysis of coagulation factors. In addition, since the activity of cathepsin G has been associated with the development of various pathological disorders, the measurement of its activity in patient samples is of high interest. Unfortunately, the usefulness of common immunological methods is limited, since they cannot distinguish between catalytically active and inactive protease. STUDY DESIGN: Here we present the application of recently developed Surface Plasmon Resonance-based biosensor for the detection of active cathepsin G in human endometrium samples. The key element of the system is based on the irreversible binding of cathepsin G to its specific phosphonic-type inhibitor immobilized on the surface of the gold chip. The concentration of cathepsin G was measured in tissue samples from the group of patients with endometriosis as well as in the control group. RESULTS: The level of cathepsin G ascertained in endometrium tissue samples was over twice as high for the group of patients suffering from endometriosis as compared to the control group, with the median values of 0.5 pmol/mg and 0.2 pmol/mg, respectively. CONCLUSION: The SPR sensor armed with a specific irreversible phosphonic inhibitor represents a highly useful tool for the determination of catalytically active cathepsin G concentration in endometrial tissue.
Cechy publikacji
publikacja naukowa oryginalna
Inne
System-identifier
PX-563242028106212707ae144b
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