Three-component EC-SPR biosensor based on graphene oxide, SiO2 and gold nanoparticles in NADH determination
PBN-AR
Instytucja
Wydział Biologiczno-Chemiczny (Uniwersytet w Białymstoku)
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
ECS Journal Solid State Science and Technology
ISSN
2162-8769
EISSN
2162-8777
Wydawca
DOI
URL
Rok publikacji
2016
Numer zeszytu
8
Strony od-do
M3015-M3018
Numer tomu
5
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
0,6
Słowa kluczowe
angielski
biosensor; electrochemical detection; gold and silica nanoparticles; graphene oxide; NADH; SPR
Streszczenia
Język
angielski
Treść
The main goal of this paper was to develop a biosensor containing three types of structures: graphene oxide (GO), silica nanoparticles (SNs) and gold nanoparticles (GNPs) for effective electrochemical and optical determination of dihydronicotinamide adenine dinucleotide dehydrogenase (NADH). The subsequent steps in biosensor formation, the manner of structure organization and the layer thickness were verified by different methods. Electrochemical oxidation of NADH was performed with the usage of differential pulse voltammetry method (DPV). Results show that obtained biosensor catalyzes the process of NADH electrochemical oxidation. The shift of oxidation potential toward less positive values and the increment of oxidation current were clearly noted. Additionally, the oxidized NAD form was found to exhibit an electrostatic affinity to the top layer of sensor giving a possibility to SPR detection. DPV and SPR signals registered for different amount of NADH were correlated with its concentration into linear relationship: y = 8.6388x − 0.0109; R2 = 0.9997 and y = 1305.8x − 74.098; R2 = 0.9913, respectively. Enhancement of the electrochemical sensitivity toward NADH, as a result of electrode modification, was proved by lower values of LOD (0.0236 mM) and LOQ (0.0707 mM) in comparison with corresponding parameters calculated for non-modified gold electrode.
Cechy publikacji
publikacja naukowa oryginalna
Inne
System-identifier
PX-578835bcc2dc0c625e1cf2f3
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