Cytotoxic interactions of bare and coated NaGdF4:Yb3+ : Er3+ nanoparticles with macrophage and fibroblast cells.
PBN-AR
Instytucja
Wydział Chemii (Uniwersytet Wrocławski)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Toxicology in Vitro
ISSN
0887-2333
EISSN
0887-2333
Wydawca
Elsevier
Rok publikacji
2016
Numer zeszytu
Strony od-do
16-25
Numer tomu
32
Identyfikator DOI
Liczba arkuszy
Autorzy
(liczba autorów: 8)
Pozostali autorzy
+ 6
Słowa kluczowe
eng
Lanthanide-doped nanocrystals
NaGdF4 nanocrystals
Nanomaterial toxicity
Cytotoxicity
Apoptosis
Proliferation
Fibroblasts
Macrophages
Streszczenia
Język
eng
Treść
The lanthanide nano-compounds are well suited to serve as fluorescent and magnetic contrast agents and luminescent labels. Although they are considered as promising materials for bio-imaging and bio-sensors in vivo or in vitro, the amount of data is still insufficient for deep understanding the toxicity of these nanomaterials. This knowledge is of great importance in the light of growing use of the biofunctionalized nanoparticles, which raises some questions about safety of these materials. Despite lanthanide-doped NaGdF4U+0026amp;nbsp;nanocrystals are considered as non-toxic, here we present the data showing the fatal effect of newly synthetized NaGdF4:Yb3U+0026amp;nbsp;+:Er3U+0026amp;nbsp;+U+0026amp;nbsp;on chosen types of cells. Our studies were performed on two cell lines NIH3T3 fibroblasts, and RAW264.7 macrophages. Cytotoxic properties of NaGdF4:Yb3U+0026amp;nbsp;+:Er3U+0026amp;nbsp;+U+0026amp;nbsp;nanoparticles and their biological effects were studied by assessing cell culture viability (MTS), proliferation and apoptosis. Bare NaGdF4:Yb3U+0026amp;nbsp;+:Er3U+0026amp;nbsp;+U+0026amp;nbsp;nanocrystals were cytotoxic and induced apoptosis of both NIH3T3 and RAW264.7 cells. Their cytotoxicity was reduced by PEGylation, at the expense of minimizing direct interactions between the compound and the cell. On the other hand, coating with silica reduced cell death induced by Yb3U+0026amp;nbsp;+:Er3U+0026amp;nbsp;+U+0026amp;nbsp;codoped NaGdF4nanocrystals (but proliferation was still inhibited). The NH2-modified silica coated nanoparticles were clearly less cytotoxic than pristine nanoparticles, which suggests that both, silica and PEG coatings are reasonable approaches to decrease cytotoxicity of the nanocrystal labels. The silica and PEG shell, should also enable and simplify further bio-functionalization of these luminescent labels. The authors acknowledge the financial support from:U+0026amp;nbsp;Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (IITD PAN)U+0026amp;nbsp;grant no.U+0026amp;nbsp;3/15,U+0026amp;nbsp;Polish Ministry of Science and Higher Education, GrantU+0026amp;nbsp;N N507 499538U+0026amp;nbsp;and from theU+0026amp;nbsp;Wroclaw Research Centre EITU+0026amp;nbsp;+U+0026amp;nbsp;within the project U+0026amp;ldquo;The Application of Nanotechnology in Advanced MaterialsU+0026amp;rdquo; U+0026amp;mdash; NanoMat (POIG.01.01.02-02-002/08) financed by the European Regional Development Fund (Operational Program Innovative Economy, 1.1.2).
Cechy publikacji
original-article
Inne
System-identifier
2016CH7657
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