Divergent expression patterns of SATB1 mRNA and SATB1 protein in colorectal cancer and normal tissues
PBN-AR
Instytucja
Wydział Lekarski (Uniwersytet Medyczny im. Piastów Śląskich we Wrocławiu)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
TUMOR BIOLOGY
ISSN
1010-4283
EISSN
1423-0380
Wydawca
SPRINGER
DOI
URL
Rok publikacji
2015
Numer zeszytu
6
Strony od-do
4441-4452
Numer tomu
36
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
SATB1 expression
colorectal cancer
normal colonic mucosa
survival
Streszczenia
Język
en
Treść
Special AT-rich sequence-binding protein 1 (SATB1) is a ‘genome organizer,’ and it has been proposed as a factor that affects the development and progression of various human neoplasms, including colorectal cancer (CRC). This study aimed to compare SATB1 expression in a group of CRC patients and healthy subjects at the mRNA and protein levels. We collected paired tumor tissue and unchanged mucosa of the large intestine from 102 CRC patients as well as 53 biopsies of normal colon mucosa obtained from healthy patients during screening colonoscopy. Tissue samples were quantified for SATB1 mRNA by quantitative PCR, while SATB1 protein expression was determined by Western blotting and immunohistochemistry. SATB1 mRNA level in tumor tissues was over twofolds lower than in samples of corresponding unchanged tissues and fourfolds lower than in biopsies of healthy colon mucosa. Western blotting analysis revealed that SATB1 protein content in tumor and unchanged tissues of CRC patients was over sixfold and fivefolds higher than in biopsies of healthy colon mucosa, respectively. Immunohistochemical staining demonstrated higher nuclear and cytoplasmic SATB1 reactivity in the tumor tissue compared to unchanged mucosa of CRC patients. Despite these differences, SATB1 mRNA, protein, and immunoreactivity levels did not correlate with patients’ clinicopathological data and their overall survival, but the latter analysis was limited by a relatively short period of follow-up. In conclusion, we suggest that some as yet unidentified posttranscriptional mechanisms that regulate SATB1 expression may be altered in the CRC tissue.
Cechy publikacji
ORIGINAL_ARTICLE
Inne
System-identifier
534191
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