Evaluation of nanoencapsulated verteporfin's cytotoxicity using a microfluidic system
PBN-AR
Instytucja
Wydział Chemiczny (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
EISSN
Wydawca
Elsevier BV
DOI
Rok publikacji
2016
Numer zeszytu
Strony od-do
39-48
Numer tomu
127
Identyfikator DOI
Liczba arkuszy
0.5
Słowa kluczowe
en
Cytotoxicity; Lab-on-a-chip; Nanoencapsulated photosensitizers; Photodynamic therapy; Verteporfin
Streszczenia
Język
en
Treść
A new-generation of nanoencapsulated photosensitizers could be a good solution to perform effective photodynamic therapy (PDT). In this paper, we present physicochemical characterization and cellular investigation of newly prepared long-sustained release oil-core polyelectrolyte nanocarriers loaded with verteporfin (nano VP) in relation to free VP. For this purpose, a macroscale multiwell plates and multifunctional microfluidic system (for three types of cell cultures: monoculture, coculture and mixed culture) were used. A physical analysis of nano VP showed its high stability, monodispersity with unimodal shape and highly positive charge, what made them good candidates for cancer treatment. Biological properties (cellular internalization and uptake as well as cytotoxicity) of nano and free VP were evaluated using both carcinoma (A549) and normal (MRC-5) human lung cells. It was investigated that verteporfin was accumulated in cancer cells preferentially. Low cytotoxicity of the tested photosensitizer was observed in both macro, and microscale. However, in experiments performed in the microsystem, nano VP allowed the reduction of cytotoxic effect, especially in relation to the normal cells. It could result from the specific environment of cell growth in the microsystem which can quite closely mimic the in vivo conditions. Our results suggest that the presented microsystem could be a very useful microtool for testing of new generation of photosensitizers in various configurations of cell cultures, which are difficult to perform in the macroscale. Moreover, the prepared nano VP could be successfully used for further research i.e. evaluation of PDT procedures.
Inne
System-identifier
WUT5c46679c7bf344a4bcb0ca1b2791e4f6
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