Liquid perfluorochemical-supported hybrid cell culture system for proliferation of chondrocytes on fibrous polylactide scaffolds
PBN-AR
Instytucja
Wydział Inżynierii Chemicznej i Procesowej (Politechnika Warszawska)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Bioprocess and Biosystems Engineering
ISSN
1615-7591
EISSN
Wydawca
DOI
URL
Rok publikacji
2014
Numer zeszytu
9
Strony od-do
1707-1715
Numer tomu
37
Identyfikator DOI
Liczba arkuszy
0.5
Autorzy
Pozostali autorzy
+ 2
Słowa kluczowe
en
liquid/liquid culture system, perfluorochemical (fluorocarbon), polylactide (PLA), fibrous scaffold, electrospinning, chondrocytes
Streszczenia
Język
en
Treść
CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed on a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as a new way of cartilage implant development. Robust and intensive growth of CP5 cells was achieved in our hybrid liquid-solid-liquid culture system consisting of the fibrous PLA scaffolds in contrast to limited growth of the CP5 cells in traditional culture system with PLA scaffold placed on solid surface. The multicellular aggregates of CP5 cells covered the surface of PLA scaffolds and the chondrocytes migrated through and overgrew internal fibers of the scaffolds. Our hybrid culture system simultaneously allows the adhesion of adherent CP5 cells to fibers of PLA scaffolds as well as, due to use of phase of PFC, enhances the mass transfer in the case of supplying/removing of respiratory gases, i.e., O-2 and CO2. Our flexible (independent of vessel shape) system is simple, ready-to-use and may utilize a variety of polymer-based scaffolds traditionally proposed for implant development.
Inne
System-identifier
WUT26101688ddc04e97afe036a5e50ff2ca
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