The effect of valproate (VPA) treatment on inositol phosphates (IPs) accumulation in non- stimulated and GnRH-treated female rat anterior pituitary cells in vitro
PBN-AR
Instytucja
Wydział Kosmetologii (Wyższa Szkoła Zawodowa Kosmetyki i Pielęgnacji Zdrowia w Warszawie)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Neuroendocrinology Letters
ISSN
0172-780X
EISSN
Wydawca
MAGHIRA & MAAS PUBLICATIONS
DOI
URL
Rok publikacji
2013
Numer zeszytu
4
Strony od-do
302-308
Numer tomu
34
Identyfikator DOI
Liczba arkuszy
1,00
Słowa kluczowe
en
VPA
GnRH
PMA
LH
IPs accumulation in rat pituitary cells
Streszczenia
Język
en
Treść
OBJECTIVE: Mechanism(s) responsible for VPA-induced effects on reproductiveaxis activity are not fully recognized. Previously we reported that VPA suppressedonly GnRH-stimulated but not the basal LH release from rat anterior pituitary(AP) cells in vitro. Since the inhibitory effect of VPA was exerted only in GnRHactivatedcells, potential VPA impact on GnRH-R-coupled IP3/PKC signalingcould not be excluded. In this study the effect of VPA on IPs synthesis in nonstimulatedand GnRH-treated rat AP cells was examined. MATERIAL AND METHODS: In the first experiment 5 × 105 cells/ml were incubatedfor 3h with VPA (10 nM–10 μM), PMA (100 nM), GnRH (100 nM), PMA(100 nM) + VPA (10 nM–10 μM), GnRH (100 nM) + VPA (10 nM–10 μM). In thesecond experiment cells were preincubated for 24h with 1μCi myo-[23 H]-inositol,then for 30 min with 10 mM LiCl and finally for 3hr with GnRH (100 nM) VPA(1 μM, 10 μM), GnRH (100 nM) + VPA (1 μM, 10 μM). LH concentration wasmeasured by RIA and intracellular IPs accumulation by ion-exchange chromatographyanalysis. RESULTS: VPA diminished GnRH-stimulated LH release without affectingPMA-induced LH release at any dose tested. Moreover, VPA-induced increase ofIPs accumulation occurred in both non- stimulated and GnRH-treated cells andintensity of cellular response was similar in both groups. CONCLUSION: VPA affects IP3/PKC pathway activity through its up-regulatoryeffect on IPs synthesis in AP cells. VPA-induced inhibition of GnRH-stimulatedLH release from gonadotrope cells appears to be the result of still unrecognizedcellular mechanism.
Cechy publikacji
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:626747