Thymidine 5'-O-monophosphorothioate induces HeLa cell migration by activation of the P2Y6 receptor
PBN-AR
Instytucja
Wydział Biotechnologii i Nauk o Żywności (Politechnika Łódzka)
Informacje podstawowe
Główny język publikacji
en
Czasopismo
Purinergic Signalling
ISSN
1573-9538
EISSN
Wydawca
DOI
URL
Rok publikacji
2016
Numer zeszytu
2
Strony od-do
199-209
Numer tomu
12
Identyfikator DOI
Liczba arkuszy
Autorzy
Słowa kluczowe
en
P2Y receptors
P2Y6
nucleoside 5′-O-monophosphorothioates
HeLa cells
cell migration
Streszczenia
Język
en
Treść
ATP, ADP, UTP, and UDP acting as ligands of specific P2Y receptors activate intracellular signaling cascades to regulate a variety of cellular processes, including proliferation, migration, differentiation, and cell death. Contrary to a widely held opinion, we show here that nucleoside 5′-O-monophosphorothioate analogs, containing a sulfur atom in a place of one nonbridging oxygen atom in a phosphate group, act as ligands for selected P2Y subtypes. We pay particular attention to the unique activity of thymidine 5′-O-monophosphorothioate (TMPS) which acts as a specific partial agonist of the P2Y6 receptor (P2Y6R). We also collected evidence for the involvement of the P2Y6 receptor in human epithelial adenocarcinoma cell line (HeLa) cell migration induced by thymidine 5′-O-monophosphorothioate analog. The stimulatory effect of TMPS was abolished by siRNA-mediated P2Y6 knockdown and diisothiocyanate derivative MRS 2578, a selective antagonist of the P2Y6R. Our results indicate for the first time that increased stability of thymidine 5′-O-monophosphorothioate as well as its affinity toward the P2Y6R may be responsible for some long-term effects mediated by this receptor.
Cechy publikacji
discipline:Biologia medyczna
discipline:Nauki farmaceutyczne
discipline:Medical biology
discipline:Pharmacy
Original article
Original article presents the results of original research or experiment.
Oryginalny artykuł naukowy
Oryginalny artykuł naukowy przedstawia rezultaty oryginalnych badań naukowych lub eksperymentu.
Inne
System-identifier
PBN-R:738437