Recombination-based generation of the agroinfectious clones of Peanut stunt virus
PBN-AR
Instytucja
Instytut Ochrony Roślin - Państwowy Instytut Badawczy
Informacje podstawowe
Główny język publikacji
angielski
Czasopismo
JOURNAL OF VIROLOGICAL METHODS
ISSN
0166-0934
EISSN
Wydawca
ELSEVIER SCIENCE BV
DOI
URL
Rok publikacji
2016
Numer zeszytu
237
Strony od-do
179-186
Numer tomu
19
Link do pełnego tekstu
Identyfikator DOI
Liczba arkuszy
Słowa kluczowe
en
Agrobacterium tumefaciens; Isothermal recombination; Peanut stunt virus; Seamless recombination; Viral infectious clones; cDNA
Streszczenia
Język
en
Treść
Full-length cDNA clones of Peanut stunt virus strain P (PSV-P) were constructed and introduced into Nicotiana benthamiana plants via Agrobacterium tumefaciens. The cDNA fragments corresponding to three PSV genomic RNAs and satellite RNA were cloned into pGreen binary vector between Cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator employing seamless recombinational cloning system. The plasmids were delivered into A. tumefaciens, followed by infiltration of hosts plants. The typical symptoms on systemic leaves of infected plants similar to those of wild-type PSV-P were observed. The presence of the virus was confirmed by means of RT-PCR and Western blotting. Re-inoculation to N. benthamiana, Phaseolus vulgaris, and Pisum sativum resulted in analogous results. Generation of infectious clones of PSV-P enables studies on virus-host interaction as well as revealing viral genes functions.
Inne
System-identifier
PX-58a2d0a6d5def2b3f7fa4e8e